The presence of hormones, drugs and chemicals in the environment are disrupting the ecosystem. The existence of chemicals in the environment is a threat to the ecosystem as they have dangerous effects on animals, plants and microbes. Progesterones are steroid hormones used for human contraception and therapeutic purposes as well as promoting animal growth. The cases of consumption of progesterone for medical purposes are much higher than estrogens, however, much studies related to estrogens have been conducted thereby neglecting the effects of progesterone. This invention focuses on removal of progesterone using fungal strain. Aspergillus niger NAAC efficiently degraded the progesterone content and transformed it into non-toxic end product. The uniqueness of this study involves preparation of a formulated product which would store the fungal strain and maintain its viability. The bioformulation was prepared using used vegetable cooking oil mixed with water as carrier. The bioformulation would reduce the efforts required to isolate the microorganism for regular usage and can be commercialized for large scale applications. To determine the storage conditions of bioformulation, various parameters were analysed which showed that storing the formulation in air tight container at room temperature would result in maximum longevity of the efficient strain.

The aim of this study was to isolate and select salt tolerant bacteria having both functions in phosphorus solubilization and phosphatase synthesis from rice-shrimp farming soil in saline areas in Mekong River Delta of Vietnam. Phosphorus solubilizing bacteria were isolated on National Botanical Research Institute's Phosphate (NBRIP) agar medium containing 1% NaCl and the activity of phosphatase enzyme was determined by disodium p-nitrophenyl phosphate reagent method at a wavelength of 420 nm. The result showed that from 15 saline soil samples, a total of 95 strains of phosphorus solubilizing bacteria were isolated and 19 of them showed their good phosphorus solubilization. The results about phosphatase activities of these 19 strains illustrated that TBT5-3 bacterial strain was the highest phosphatase producing strain with an amount of 0.377 U/mL after 10 days of incubation. This strain showed its best phosphatase producing capacity when cultured in the liquid culture medium containing pH 5, 1% NaCl, glucose and urea under the shaking speed of 120 rpm. Based on 16S rRNA gene sequence analysis this phosphatase synthesizing bacterial strain was genetically identified as species of Bacillus sp. TBT5-3 since 100% of this train sequence is affiliated with Bacillus megaterium.

Technology has been playing a very important role in the advancement of science, and big breakthroughs in science are dependent upon technologies available from time to time. Between science and technology, a good understanding is required so that available technologies can be used most efficiently and effectively to solving the targeted questions in scientific research. Genome nucleotide sequencing as an available technology after several generations of the technical improvement is capable of extract the full nucleotide sequences of any single organism or a mixed population of organisms in a given sample. This technology has a major important role in medicine and health science for diagnosis of the microorganisms and early detection of genetic diseases. In microbial ecology and applied microbiology, it is now fashionable to sequence the microbial community, the microbiome, so that the community composition and diversity are described from air, soil, water, sludge to skin and gut of animals, including humans. This routine practice, too frequently used by far too many, neither provide any significant insights of knowledge nor unravel the fundamental science in any way, but it serves as a cosmetic decoration for publication mostly. Because of this, not sequencing or sequencing less is the view expressed here to alert researchers for a better focus on the research questions, and the selection and implementation of the necessary physical science methods to obtain the solid research data that can advance science in a significant way. Publication driven experiments to produce more papers are eroding the essence of science, especially the pure spirit of science.