https://www.udspub.com/ajj/public/index.php/aeb/issue/feedApplied Environmental Biotechnology2024-03-26T10:22:37Urban Development Scientific Publishingeditorial@udspub.comOpen Journal Systemshttps://www.udspub.com/ajj/public/index.php/aeb/article/view/1782Plastic-degrading bacteria isolated from contaminated mangrove sediment in Wonorejo, Surabaya2024-03-26T10:22:37Maya Shovitrimaya@bio.its.ac.idHefdiyah Hefdiyahhefdiyah@gmail.comTiti Rindi Antikatiti_rindi@yahoo.co.idNengah Dwianita Kuswytasarikuswytasari@bio.its.ac.idNur Hidayatul Alamihidayatulalami@bio.its.ac.idEnny Zulaikaenny@bio.its.ac.idSeung Wook Kimkimsw@korea.ac.krMin Kyu Ohmkoh@korea.ac.krPlastics have become inevitable needs in modern society due to their attractive properties, including thermostability, lightweight, flexibility, superior insulation, and low cost, which have led to massive production. Their persistence and challenges in disposal have detrimental effects on the environment leading to the development of a promising degradation process which is efficient, time-saving, and cost-effective. This study focused on discovering the potential plastic-degrading bacteria isolated from plastic-contaminated mangrove sediment in the Wonorejo area. We buried a commercially available plastic bag in the polluted mangrove sediment for 16 weeks. Our results showed that indigenous bacteria formed a biofilm on the plastic surface leading to a plastic dry weight loss of up to 12%. FTIR analysis revealed obvious transmittance attenuations in the buried plastic polymer, suggesting that their chemical properties may have been interrupted due to bacterial activity. Further, bacteria isolation and biochemical screening revealed that they were primarily dominated by Bacillus. According to 16S rRNA sequencing, they were identified as Brevibacilllus (BIO-B), Stenotrophomonas (BIO-G), and Lysinibacillus (SOI-C). The three genera mentioned earlier exhibited a detectable level of plastic-degrading activity and possessed lipolytic, ligninolytic, and alkane-degrading activities. Stenotrophomonas (BIO-G) showed a degradation activity on low-density polyethylene (LDPE) represented by a plastic dry weight loss of up to 8.9% within 4 weeks. As expected, plastic treated with BIO-G showed transmittance attenuation in FTIR analysis, albeit with a lower percentage than that treated with indigenous bacteria. Moreover, SEM analysis reveals changes in the morphological surface of plastic. Together, FTIR and SEM analysis indicated that bacteria disrupt both the chemical structure and morphological appearance of plastic polymer upon degradation process. These results denote that BIO-G indeed composes the aforementioned indigenous bacteria from polluted mangrove sediment. Thus, our study suggests the indigenous bacteria isolated from contaminated areas produced plastic-degrading enzymes and secreted to the environment to break down plastic compounds.2023-12-30T00:00::00Copyright (c) 2023 Maya Shovitri, Hefdiyah Hefdiyah, Titi Rindi Antika, Nengah Dwianita Kuswytasari, Nur Hidayatul Alami, Enny Zulaika, Seung Wook Kim, Min Kyu Ohhttps://www.udspub.com/ajj/public/index.php/aeb/article/view/1728Microplastic Contamination Analysis For Chennai Coastal Region2024-03-26T09:55:43Somasundaram Rameshparthibaba1926@gmail.comRadhakrishnan Nagalakshminagalakshmirphd23@gmail.comMicroplastic contamination in the Chennai coastal region is a growing concern due to the intake of abandoned garbage from various sources. This study aimed to assess the extent of microplastic contamination by analyzing microplastic trash collected from 25 spots along the Tamil Nadu coast, spanning a distance of 1076 kilometers. The results revealed that microplastic contamination was more prevalent during higher wave conditions compared to lower tides. Shorelines near riverbanks exhibited significantly higher quantities of microplastics than those affected by fisheries and tourist activities. The main types of microplastics found were polyethylene, polypropylene, and polystyrene, with plastic shards comprising the majority of the trash (47-50%). Furthermore, analyses of fish species collected from shore regions showed that 10.1% of the fishes had consumed plastic particles. This emphasizes the potential risk of microplastics entering the marine food chain. The study highlights the need for microplastic filtering from estuaries, coastline waters, and other potential sources. In conclusion, microplastic contamination poses a serious hazard to the Chennai coastal region. Urgent measures are required to mitigate and reduce microplastic pollution, particularly near river openings, to protect the marine ecosystem and the food chain. Efforts should focus on preventing the entry of microplastics into the environment and promoting sustainable waste management practices to safeguard the coastal ecosystem.2024-03-12T10:11:30Copyright (c) 2023 Somasundaram Ramesh, Radhakrishnan Nagalakshmihttps://www.udspub.com/ajj/public/index.php/aeb/article/view/1724Biotechnological methods in pulp and paper production from an alternative source of raw materials - the savior of the ecosystem2024-03-26T09:55:43Almira Saparbekovaalmira.saparbekova@mail.ruAlina Altekeyaltekey@mail.ruGalina Seitmagzimovagalinaseit@mail.ruAnar Esimovaesimova_anar@mail.ruDarikha Kudasovadariha_uko@mail.ruZhuldyz Ibraimovaibraymova_zhuldyz@mail.ruThe pulp and paper industry is at its peak due to the annual growth in demand for paper products which includes packaging board, hygienic paper products, printing papers and the list is endless. To date, the main source of raw materials for pulp and paper production is wood. However, its use and the use of standard flow charts in pulp and paper production creates environmental problems. For example, deforestation to obtain raw materials leads to a disruption of biogeocenoses, and technology using alkalis and acids are sources of toxic wastewater that pollute the hydrosphere and lithosphere. In order to avoid the above environmental problems, other options for sustainable sources of raw materials can be considered, such as agricultural waste - straw. By its natural origin, straw is a non-woody plant, which will significantly simplify the flow chart of pulp and paper production, on the one hand, and on the other hand, the use of straw in production will help to reduce the risks of early global warming, since most of the straw is burned in the fields. As far as toxic wastewater emissions are concerned, they can be drastically reduced through the biological degradation of straw lignin to produce pure pulp. Biodegradation of lignin can be produced by widespread wood-destroying fungi due to their ability to synthesize lignin-degrading enzymes. Thus, the use of biological agents in pulp and paper production will make it possible to eliminate the use of caustic acids and alkalis.2024-01-30T10:29:38Copyright (c) 2023 Almira Saparbekova, Alina Altekey, Zhanar Aimenova, Zhamila Baimirzaeva, Gulnur Kaldybekova