The mutagenicity of organic extracts in source water and peripheral water with different disinfection ways

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Yi Zhong, Xiao-Tong Li, Qi-Yi Huang, Ren-De Huang, Zi-Yan Zhou, Hua Bi, Peng-Ya Feng, De-Dong Wang


This study aimed to determine mutagen contamination, to compare the differences between inlet and outlet distribution, and the possible impacts on public health. Water samples were collected from four different waterworks in Guangzhou, China. The Ames test was conducted to investigate the potential mutagenicity caused by organic extracts from drinking water sources and peripheral water. Organic content was extracted with XAD-2 resin column and organic solvents, and toxicity was tested in three doses of extract equivalent, 0.2, 0.4 and 0.8 L source water. The results of the Ames test showed that all the organic extracts from water samples could induce different levels of mutagenic potentials in the absence of S9 mix, which indicated mutagenicity and strain. Comparing with TA98, TA100 was more sensitive in genotoxicity. Mutagenic enhancement factors were found in both drinking water sources and peripheral water. Water treatment technologies with different disinfection ways could increase the mutagenicity of water, but the biological significance of mutagenicity of the organic extracts remained to be further confirmed. The results suggested that it was necessary to concern the relationship between source water, water treatment unit and the mutagenicity factors of water.


mutagenicity; organic extracts; source water; peripheral water; disinfection

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Alper, H. and Stephanopoulos, G., 2009. Engineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential? Nature Reviews Microbiology, 7(10), 715-723.

Andrews, J.M., 2001. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy, 48(suppl_1), 5-16.

Anthonia, E.E. and Philip H.S., 2015. An overview of the applications of furfural and its derivatives. Int J Adv Chem., 3, 42-47.

Anto, R.J., Kuttan, G., Babu, K.V.D., Rajasekharan, K.N. and Kuttan, R., 1998. Anti‐inflammatory activity of natural and synthetic curcuminoids. Pharmacy and Pharmacology Communications, 4(2), 103-106.

Atherton, F.R., Hassall, C.H. and Lambert, R.W., 1986. Synthesis and structure-activity relationships of antibacterial phosphonopeptides incorporating (1-aminoethyl) phosphonic acid and (aminomethyl) phosphonic acid. Journal of medicinal chemistry, 29(1), 29-40. 10.1002/chin.198620249

Atlas, R.M., 1998. Microbial Ecology:Fundamentals And Applications, 4/E, Pearson Education India.

Bridgwater, A.V., 2003. "Renewable fuels and chemicals by thermal processing of biomass." Chemical Engineering Journal, 91(2), 87-102.

Cabana, H., Jones, J., and Agathos, S.N., 2007. Elimination of endocrine disrupting chemicals using white rot fungi and their lignin modifying enzymes: a review. Engineering in Life Sciences, 7(5), 429-456.

Capello, C., Fischer, U. and Hungerbühler, K., 2007. What is a green solvent? A comprehensive framework for the environmental assessment of solvents. Green Chemistry, 9(9), 927-934. 10.1039/b617536h

Cherubini, F., 2010. The biorefinery concept: using biomass instead of oil for producing energy and chemicals. Energy conversion and management, 51(7), 1412-1421.

Fu, J., Cheng, K. and Zhu, H.L., 2010. Synthesis, structure and structure–activity relationship analysis of caffeic acid amides as potential antimicrobials. European journal of medicinal chemistry, 45(6), 2638-2643.

Galletti, P.A., Montecavalli, A., Moretti, F., Pasteris, A., Samorì, C. and Tagliavini, E., 2009. Furan containing ammonium salts from furfural: synthesis and properties evaluation. New Journal of Chemistry, 33(9), 1859-1868. 10.1039/B902855B

Ganushchak, N., Lesyuk, A.I., Fedorovich, I.S., Obushak, N.D. and Murarash, M.M., 2001. Synthesis and Transformations of Derivatives and Analogues of α‐Cyanocinnamic Acid. Russian journal of organic chemistry, 36(11), 1677-1682.

Moreno, G., Trampuz, M.A. and Di Luca, M., 2017. Synergistic antibiotic activity against planktonic and biofilm-embedded Streptococcus agalactiae, Streptococcus pyogenes and Streptococcus oralis. Journal of antimicrobial Chemotherapy, 72(11), 3085-3092. 10.1093/jac/dkx265

Green III, F. and Highley, T.L., 1997. Mechanism of brown-rot decay: paradigm or paradox. International Biodeterioration & Biodegradation, 39(2), 113-124. 10.1016/s0964-8305(96)00063-7

Gu, J.D., 2003. Microbiological deterioration and degradation of synthetic polymeric materials: recent research advances. International Biodeterioration & Biodegradation, 52, 69-91. 10.1016/S0964-8305(02)00177-4

Higginbotham, K.L., K.P. Burris, Zivanovic, S., Davidson, P.M. and Stewart, C.N., 2014. Aqueous extracts of Hibiscus sabdariffa calyces as an antimicrobial rinse on hot dogs against Listeria monocytogenes and methicillin-resistant Staphylococcus aureus. Food Control, 40, 274-277. 10.1016/j.foodcont.2013.12.011

Hyde, S.M. and Wood, P.M., 1997. A mechanism for production of hydroxyl radicals by the brown-rot fungus Coniophora puteana: Fe (III) reduction by cellobiose dehydrogenase and Fe (II) oxidation at a distance from the hyphae. Microbiology, 143(1), 259-266.

Itoh, K. and Yatome, C., 2004. Decolorization and degradation of xanthene dyes by a white rot fungus, Coriolus versicolor. Journal of Environmental Science and Health, Part A 39(9), 2383-2389.

Kabara, J.J. and Conley, A.J., 1972. Relationship of chemical structure and antimicrobial activity of alkyl amides and amines. Antimicrobial Agents and Chemotherapy, 2(6), 492-498. 10.1128/AAC.2.6.492

Kamm, B. and Kamm, M., 2004. Principles of biorefineries. Applied microbiology and biotechnology, 64(2), 137-145.

Keharia, H. and Madamwar, D., 2002. Transformation of textile dyes by white-rot fungus Trametes versicolor. Applied Biochemistry and Biotechnology, 102(1-6), 99-108.

Leeds, J.A., Schmitt, E.K. and Krastel, P., 2006. Recent developments in antibacterial drug discovery: microbe-derived natural products–from collection to the clinic. Expert opinion on investigational drugs, 15(3), 211-226. 10.1517/13543784.15.3.211

Liang, X., Duan, Y., Wang, J. and Zhou, M., 2016. Photochemical degradation of bismerthiazol: structural characterisation of the photoproducts and their inhibitory activities against Xanthomonas oryzae pv. oryzae. Pest management science, 72(5), 997-1003.

Mamedov, V., Valeeva, V., Antokhina, L.A., Chernova, A.V., Shagidullin, R.R., Doroshkina, G.M., Nuretdinov, I.A., 1994. Darzens synthesis of 2, 2-dichloro-3-(2-furyl)-3-hydroxypropionic acid derivatives. Russian chemical bulletin, 43(8), 1368-1372.

Millati, R., Syamsiah, S., Niklasson, C., Cahyanto, M.N., Ludquist, K., Taherzadeh, M.J., 2011. Biological pretreatment of lignocelluloses with white-rot fungi and its applications: a review. Bioresources, 6(4), 5224-5259.

Mir-Tutusaus, J.A., Masís-Mora, M., Corcellas, C., Eljarrat, E., Barceló, D., Montserrat, S., Caminal, G., Vicent, T., Rodríguez-Rodríguez, C.E., 2014. Degradation of selected agrochemicals by the white rot fungus Trametes versicolor. Science of the Total Environment, 500, 235-242.

Patch, J.A. and Barron, A.E., 2003. Helical peptoid mimics of magainin-2 amide. Journal of the American Chemical Society, 125(40), 12092-12093.

Schelz, Z., Molnar, J. and Hohmann, J., 2006. Antimicrobial and antiplasmid activities of essential oils. Fitoterapia, 77(4), 279-285.

Schulz, S. and Dickschat, J.S., 2010. Biological activity of volatiles from marine and terrestrial bacteria. Marine drugs, 8(12), 2976-2987.

Shah, V. and Nerud, F., 2002. Lignin degrading system of white-rot fungi and its exploitation for dye decolorization. Canadian journal of microbiology, 48(10), 857-870.

Sun, Y. and Sun, G., 2001. Novel regenerable N‐halamine polymeric biocides. I. Synthesis, characterization, and antibacterial activity of hydantoin‐containing polymers. Journal of Applied Polymer Science, 80(13), 2460-2467. 10.1002/app.1353

Tang, R., Jin, L., Mou, C., Yin, J., Bai, S., Hu, D., Wu, J., Yang, S., and Song, B., 2013. Synthesis, antifungal and antibacterial activity for novel amide derivatives containing a triazole moiety. Chemistry Central Journal, 7(1), 30.

Weilmuenster, E.A. and Jordan, C.N., 1945. The Preparation and Properties of Some Furfuryl Quaternary Ammonium Compounds1, 2. Journal of the American Chemical Society, 67(3), 415-416.

Yaman, S., 2004. Pyrolysis of biomass to produce fuels and chemical feedstocks. Energy conversion and management, 45(5), 651-671.



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