DECOLORIZATION, DEGRADATION, AND DETOXIFICATION OF REACTIVE TEXTILE DYES BY INDIGENOUSLY ISOLATED BACTERIA FROM DYE CONTAMINATED SITES FOR ENVIRONMENTAL CLEANUP

Kumar Sakthishabarish; Krishnan Kannabiran

doi:10.26789/AEB.2024.02.012

DECOLORIZATION, DEGRADATION, AND DETOXIFICATION OF REACTIVE TEXTILE DYES BY INDIGENOUSLY ISOLATED BACTERIA FROM DYE CONTAMINATED SITES FOR ENVIRONMENTAL CLEANUP

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Kumar Sakthishabarish, Krishnan Kannabiran

Abstract

Textile industries are the second-largest polluting sector in contaminating soil and water resources. Freshwater depletion demands the recycling and reuse of industrial wastewater. This study aimed for microbial treatment of dye-contaminated water for agricultural applications. In this study, potential microbes were isolated and screened based on a maximum dye tolerance test, decolorization, and degradation studies against four reactive textile dyes (Black B, Red GDN, Black NN, and Yellow GDBR). The potential isolate was identified as Pseudomonas aeruginosa strain VITKSS1. Decolorization of the reactive dye mix at 100 ppm, 500 ppm, and 1000 ppm was observed as 100% in 7 days, 94.4% in 8 days, and 85.9% in 10 days respectively. Bio-degraded dye metabolites were analyzed using GC-MS and FTIR. Based on the GC-MS analysis, an 87.85% reduction of Bis-(2-ethylhexyl)-phthalate (DEPH) was observed. The surface of the degraded dye metabolites was visualized in SEM. The isolate VITKSS1 showed positive results on plant growth-promoting rhizobacteria (PGPR) screening. The phytotoxicity study was performed to assure the safety of treated wastewater for irrigation. This study revealed that dye-contaminated water can be treated using VITKSS1 and reused efficiently.

Keywords

Keywords: Synthetic dyes, bioremediation, microbial degradation, Pseudomonas aeruginosa, and phytotoxicity.

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DOI: https://doi.org/10.26789/AEB.2024.02.012

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