Antibacterial and antioxidant activity of green synthesized Zinc oxide nanoparticles using polyphenol extract from Mentha piperita seeds

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AbdulAziz Mohamed Gad, Roqaya I. Bassuiny, Eman R. Zaki, Mohamed A. Yassin, Mona Wahdan, Emad Tolba


Currently green nanotechnology presents a smart solution to produce novel nanostructured materials that are highly safe and environmental friendly. In this work, zinc oxide nanoparticles (ZnO NPs) were prepared by employing aqueous mint (M. piperita) seeds extract at 60 oC, as a green synthesis method. Mint seeds extract was chosen among the 6 plant seeds that were the subject of this study due to it represents the highest content of polyphenols and flavonoids, as well as antioxidant activity. High Performance Liquid Chromatography (HPLC) showed that syringic acid (16%), rutin (22%), and apigenin-7-O-glucoside (29%) were the main component of ethanol mint seeds extract.  The produced ZnO NPs were examined using an ultraviolet-visible spectrophotometer (UV-VIS), X-ray diffraction (XRD) and transmission electron microscope (TEM). The UV spectrum revealed maximum absorption value at 376 nm, related to green synthesized ZnO NPs. The XRD study demonstrated the creation of ZnO NPs. ZnO NPs were spherical in shape with average particles size of 80±36 nm. The antibacterial activity of aqueous mint seeds extract and green synthesized ZnO NPs were investigated on E. coli and S. aureus and antioxidant activity as well. Our findings demonstrate a facile approach to improve the antibacterial potential of the ZnO NPs, and therefore could be a promising multifunctional bioactive material for wound healing and other related applications.


flavonoids, HPLC, Mint, aqueous extract, syringic acid, rutin

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