Isolation, Screening, and Molecular Characterization of Rhizosphere Derived Potential Biofertilizer from Different Crops Land for Sustainable Agriculture and Environment

Abstract

Overpopulation in world has created immense pressure on agricultural land to increase crops production by using excess chemical fertilizers, pesticides, and plant growth regulators. Nowadays, biofertilizer is one of the best concerns of research interest for sustainable development in agriculture and environment. About 30 soil bacteria were isolated from three different locations in Bangladesh by growing on nitrogen-free selective media and primarily had been categorized based on colony features. However, to short out the biofertilizer and biocontrol potential bacteria several assay had been conducted including nitrogen-fixing assay, ammonia production assay, phosphate solubilizing assay, IAA production assay, siderophores production assay, amylase production assay, biocontrol assay, and seed germination bioassay. Different species of Rhizobium, Bacillus, Paraburkholderia, Priestia, Arthrobacter, Pseudarthrobacter, Rhodanobacter, Flavobacterium, Mucilaginibacter, and Sphingomonas were identified by analyzing 16S rRNA gene partial sequence analysis by BLASTn that revealed many of these act as biofertilizer. Moreover, the antibiotic resistance tests of biofertilizer potential isolates and all others intensive data analysis in respect of biofertilizer functionality revealed that Paraburkholderia sacchari is the best while Priestia megaterium, Bacillus zanthoxyli, Arthrobacter globiformis, and Bacillus wiedmanni have also the potentiality to be biofertilizer. The outcome will help the researchers and industries to develop biofertilizer for crops plant.