Biotoxicity of NH4+ on Nostoc Sphaeroides (diazotrophic cyanobacteria) in paddy floodwater

Jihai Shao; Li Fan; Ye Feng

doi:10.26789/AEB.2023.01.005

Biotoxicity of NH4+ on Nostoc Sphaeroides (diazotrophic cyanobacteria) in paddy floodwater

VIEWS - 182 (Abstract) 71 (PDF)

Jihai Shao, Li Fan, Ye Feng

Abstract

In order to elucidate the toxicity of NH₄⁺ on Nostoc Sphaeroides (commonly named GE-XIAN-MI in China, GXM), a diazotrophic cyanobacterium in paddy fields, this study investigated the growth, photosynthesis, N₂-fixation, and oxidative-antioxidative characteristics of GXM under NH₄⁺stress in paddy floodwater. The results showed that the photosynthesis and the N₂-fixing ability of GXM were inhibited by NH₄⁺ at a concentration of 13.46 mg L^-1 in paddy floodwater. The main target of NH₄⁺ on photosystem II (PS II) of GXM was the electron donor side of react center. Oxygen evolution complex (OEC) was the secondary target of toxicity of NH₄⁺ on PS II. The damage of PS II led to accumulation of ROS and resulted in oxidative damage on plasma membrane of GXM. Feedback inhibition and decrease of photosynthetic energy production may be the reasons for the decrease of N₂-fixing ability of GXM under NH₄⁺ stress. The results presented in this study suggest that high level of NH₄⁺ loading may be a reason for the recession of GXM resource in paddy fields.

Keywords

Nostoc Sphaeroides; Ammonium; Paddy floodwater; Photosynthesis; Nitrogenase; Oxidative damage

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DOI: https://doi.org/10.26789/AEB.2023.01.005

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