Antibiotic Susceptibility and Differential expression of virulence genes in Staphylococcus aureus

VIEWS - 370 (Abstract) 66 (PDF)
Mohammad A. Alkafaween, Mohammad Abu-Sini, Hamid A. Nagi Al-Jamal


Staphylococcus aureus is one of the most common pathogens in biofilm-associated chronic infections. S. aureus that live within biofilms avoid the host's immune response and are more resistant to antibiotics than planktonic bacteria. The current study was conducted to evaluate the antibacterial, antibiofilm and antivirulence of seven antibiotics (Ciprofloxacin (CP), Gentamicin (GEN), Tetracycline (TET), Amikacin (AMK), Clindamycin (CLI), Erythromycin (Ery) and Vancomycin (VAN) against S. aureus. The effects of seven antibiotics (CP, AMK, VAN, TET GEN, Ery and CLI) on S. aureus planktonic and biofilm were determined via Antibiotic susceptibility test, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), disruption of microcolony, biofilm inhibition and degradation (crystal violet staining) and RT-qPCR. Antibiotic susceptibility test showed that CP, AMK, VAN, TET GEN, Ery and CLI has antibacterial activity against S. aureus with an inhibition zone of 28 mm, 21 mm, 27 mm, 20 mm, 25 mm, 27 mm and 19 mm respectively. The results showed that CP and AMK possessed the lowest MIC value against S. aureus with 0.125 µg/mL and 0.25 µg/mL for VAN, TET and GEN and 1.0 for Ery and CLI. The recorded values for MBCs were 0.25 μg for CP and AMK for S. aureus, 0.5 μg for vancomycin, tetracycline and gentamicin for S. aureus and 1.0 μg for Ery and CLI for S. aureus. Notably, CP and AMK demonstrated considerable efficacy, as shown by the low values for MIC; 0.125 μg and MBC; 0.25 μg for S. aureus. All antibiotics were found to disrupt microcolony formation in S. aureus at MIC of each antibiotics. At 0.25 μg concentration to 8 μg concentration of each antibiotic were significantly found to degrade and inhibit biofilm formation of S. aureus. The RT-qPCR showed that four genes including argF, purC, adh, and fabG were downregulated, whilst, three genes including scdA, pykA and menB were upregulated after exposure to CP, AMK, VAN, TET GEN, Ery and CLI. This study showed the efficacy of seven antibiotics against planktonic, biofilm, gene expression and that different concentrations of antibiotics have different degrees of potential effect on established biofilm. In addition, a decreased expression of virulence genes in S. aureus will impact their pathogenicity. These results provide the theoretical parameters for the selection of effective antimicrobial in clinical therapy and demonstrate how to correctly use antibiotics at MIC and sub-MIC as preventive drugs.


Staphylococcus aureus; Antibiotics, Biofilm; Differential expression; RT-qPCR; Virulence genes.

Full Text:





  • There are currently no refbacks.

Copyright (c) 2022 Mohammad A. Alkafaween et al.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Cookies Notification