A thermophilic nitrate-reducing bacterium isolated from production water of a high temperature oil reservoir and its inhibition on sulfate-reducing bacteria

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Jin-Feng Liu, Wei-Lin Wu, Feng Yao, Biao Wang, Bing-Liang Zhang, Serge Maurice Mbadinga, Ji-Dong Gu, Bo-Zhong Mu


A thermophilic spore-forming facultative anaerobic bacterium, designated as Njiang2, was isolated from the production water of a high temperature oil reservoir (87°C). The physiological, biochemical and 16S rRNA gene based phylogenetic analysis indicated that Njiang2 belonged to the genus Anoxybacillus. Njiang2 could significantly inhibit H2S production when co-cultured with Desulfotomaculum sp under laboratory conditions, which implied its great potential in mitigation of brine souring in the oil reservoir and in control of biocorrosion caused by sulfate-reducing bacteria. As far as we know, this might be the first report of Anoxybacillus sp. isolated from high temperature oilfield


Anoxybacillus; thermophilic; 16S rRNA gene analysis; nitrate-reducing bacterium;souring mitigation; mi-crobial influenced corrosion

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DOI: http://dx.doi.org/10.18063/AEB.2016.02.004


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