Bioethanol Production by Immobilization, Growth Variables, and Co-cultivation of the Yeast Meyerozyma Guilliermondii and the Plant Zea Mays (Sweet Corn)

Mustafa R. AL-Shaheen; Ahmad Kamis; Ali Hazim Abdulkareem

doi:10.26789/AEB.2025.01.002

Bioethanol Production by Immobilization, Growth Variables, and Co-cultivation of the Yeast Meyerozyma Guilliermondii and the Plant Zea Mays (Sweet Corn)

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Mustafa R. AL-Shaheen, Ahmad Kamis, Ali Hazim Abdulkareem

Abstract

The increasing demand for renewable energy has driven research into bioethanol production. This study explores the use of immobilized Meyerozyma guilliermondii yeast and co-cultivation with Zea mays (sweet maize) to enhance bioethanol yields. Optimal conditions for yeast adhesion to alginate beads and co-cultivation effects on ethanol production were investigated. Variables such as alginate concentration, bead size, temperature, pH, and nutrients were adjusted to maximize yeast viability and sugar availability. Co-cultivation demonstrated synergistic benefits, significantly improving bioethanol output compared to traditional methods. This approach offers sustainable, efficient bioethanol production with promising environmental and economic advantages.

Keywords

Bioethanol; Immobilization; Yeast; Co-cultivation fermentation

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References

Al-Shaheen, M.R., Hamad, R.M., Abdaly, M.M.A., Al-Rawi, O.H., 2020. Assessment the impact of iron nanoparticles and dry yeast extract on the corn (Zea maize L.). Journal of Physics: Conference Series, 1535(1): 012052.

https://doi.org/10.1088/1742-6596/1535/1/012052

Al-Shaheen, M.R., Zakaria, Z., Al-Shaheen, M.R., 2022. A review about the bioactivity of a stevia extract to inhibit the effectiveness of some types of UTI bacteria. AIP Conference Proceedings, 2400(1).

https://doi.org/10.1063/5.0112570

Barroso-Solares, S., Lopez-Moya, F., Fraile, T., Prieto, Á. C., Lopez-Llorca, L., Pinto, J., 2025. Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 334: 125928.

https://doi.org/10.1016/j.saa.2025.125928

Cherwoo, L., Gupta, I., Flora, G., Verma, R., Kapil, M., Arya, S.K., Ashokkumar, V., 2023. Biofuels an alternative to traditional fossil fuels: a comprehensive review. Sustainable Energy Technologies and Assessments, 60: 103503.

https://doi.org/10.1016/j.seta.2023.103503

Du, R., Guo, W., Shen, Y., Dai, J., Zhang, H., Fu, M., Wang, X., 2023. In situ assay of the reducing sugars in hydrophilic natural deep eutectic solvents by a modified DNS method. Journal of Molecular Liquids, 385: 122286.

https://doi.org/10.1016/j.molliq.2023.122286

Herrera-Balandrano, D.D., Wang, S.Y., Wang, C.X., Shi, X.C., Liu, F.Q., Laborda, P., 2023. Antagonistic mechanisms of yeasts Meyerozyma guilliermondii and M. caribbica for the control of plant pathogens: A review. Biological Control, 186: 105333.

https://doi.org/10.1016/j.biocontrol.2023.105333

Himawan, A., Anjani, Q.K., Detamornrat, U., Vora, L.K., Permana, A.D., Ghanma, R., Donnelly, R.F., 2023. Multifunctional low temperature-cured PVA/PVP/citric acid-based hydrogel forming microarray patches: Physicochemical characteristics and hydrophilic drug interaction. European Polymer Journal, 186: 111836.

https://doi.org/10.1016/j.eurpolymj.2023.111836

Li, K., Ding, C., Zhang, J., Du, B., Song, X., Wang, G., Zhang, Z., 2024. Accurate identification of methanol and ethanol gasoline types and rapid detection of the alcohol content using effective chemical information. Talanta, 274: 125961.

https://doi.org/10.1016/j.talanta.2024.125961

Liu, X., Yu, X., He, A., Xia, J., He, J., Deng, Y., Zhao, P., 2022. One-pot fermentation for erythritol production from distillers grains by the co-cultivation of Yarrowia lipolytica and Trichoderma reesei. Bioresource Technology, 351: 127053.

https://doi.org/10.1016/j.biortech.2022.127053

Malik, K., Salama, E.S., El-Dalatony, M.M., Jalalah, M., Harraz, F.A., Al-Assiri, M.S., Li, X., 2021. Co-fermentation of immobilized yeasts boosted bioethanol production from pretreated cotton stalk lignocellulosic biomass: Long-term investigation. Industrial Crops and Products, 159: 113122.

https://doi.org/10.1016/j.indcrop.2020.113122

Patel, K., and Singh, S.K., 2024. Environmental sustainability, energy efficiency and uncertainty analysis of agricultural residue-based bioethanol production: A comprehensive life cycle assessment study. Biomass and Bioenergy, 191: 107439.

https://doi.org/10.1016/j.biombioe.2024.107439

Popović, N., Pržulj, D., Mladenović, M., Prodanović, O., Ece, S., Đurđić, K.I., Prodanović, R., 2021. Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization. International Journal of Biological Macromolecules, 181: 1072-1080.

https://doi.org/10.1016/j.ijbiomac.2021.04.115

Sakthishabarish, K., and Kannabiran, K., 2025. Decolorization, degradation, and detoxification of reactive textile dyes by indigenously isolated bacteria from dye contaminated sites for environmental cleanup. Evaluation, 76: 86.

https://doi.org/10.26789/AEB.2024.02.012

Sidana, A., Kaur, S., and Yadav, S.K., 2023. Assessment of the ability of Meyerozyma guilliermondii P14 to produce second-generation bioethanol from giant reed (Arundo donax) biomass. Biomass Conversion and Biorefinery, 13(18): 16723-16735.

https://doi.org/10.1007/s13399-021-02211-4

Wang, X., Li, W., Mahsa, G.C., Zhang, C., Ma, K., Rui, X., Li, W., 2023. Co-cultivation effects of Lactobacillus helveticus SNA12 and Kluveromyces marxiensis GY1 on the probiotic properties, flavor, and digestion in fermented milk. Food Research International, 169: 112843.

https://doi.org/10.1016/j.foodres.2023.112843

Yan, W., Zhang, L., Lin, F., He, S., 2021. Biotechnological applications of the non-conventional yeast Meyerozyma guilliermondii. Biotechnology Advances, 46: 107674.

https://doi.org/10.1016/j.biotechadv.2020.107674

Zoghi, A., Todorov, S.D., and Khosravi-Darani, K., 2024. Bio-detoxification of mycotoxin-contaminated feedstuffs: Using lactic acid bacteria and yeast. Applied Environmental Biotechnology, 9(1): 62-75.

https://doi.org/10.26789/AEB.2024.01.007

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DOI: https://doi.org/10.26789/AEB.2025.01.002

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